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a hong kong 8 68 2e∧6 influenza b n d n d n d atcc vr 1735d  (ATCC)


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    ATCC a hong kong 8 68 2e∧6 influenza b n d n d n d atcc vr 1735d
    A Hong Kong 8 68 2e∧6 Influenza B N D N D N D Atcc Vr 1735d, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/a+hong+kong+8+68/Genomic+RNA+from+Influenza+B+virus+strain+B%2FTaiwan%2F2%2F62/us12529113-383-181-189
    Average 92 stars, based on 5 article reviews
    a hong kong 8 68 2e∧6 influenza b n d n d n d atcc vr 1735d - by Bioz Stars, 2026-09
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    Transient overexpression of CerS4 impairs productive infection of influenza viruses. ( A ) HEK293FT cells were transfected with control plasmid (−) or plasmid encoding Myc-tagged CerS4 (+). The cells were mock-infected or infected with IAV (pH1N1) at an MOI of 1 at 18 hours post-transfection. At 24 hpi, cells were harvested, and Western blotting was performed to detect the levels of IAV-HA, PB1, NS1, M1, Myc-tagged CerS4, and GAPDH. Densitometric values are shown, with relative expression of viral protein/GAPDH in vector control and infected samples set to 1.0. The effect of CerS4 overexpression in infected cells was compared. ( B ) A549 cells were transfected with vector control or CerS4-encoding plasmid. At 18 hours after transfections, cells were mock-infected or infected with pH1N1 at an MOI of 3 for 8 hours. Expression levels of viral proteins were assessed as shown in panel ( A ). ( C–E ) A549 cells were transfected with a vector control plasmid (Control) or plasmid encoding CerS4 (CerS4) or plasmid encoding CerS1 (CerS1, only). In ( C ), at 18 hours post-transfection, the cells were infected with IAV pH1N1 at an MOI of 0.01. At 1 ( n = 7/group), 2 ( n = 3/group), or 3 ( n = 6/group) dpi, cellular supernatants were harvested. Control vs CerS1 and Control vs CerS4 titers were statistically analyzed as indicated. In ( D ), A549 cells were infected with IAV pH1N1 at an MOI of 3 for 8 hours, followed by supernatant collection for titration by plaque assay ( n = 3/group). In ( E ), A549 cells were infected with IAV WSN at an MOI of 0.001. Cell supernatants were collected at 2 and 3 dpi for assessing viral titers using plaque assay on MDCK cells ( n = 3/group). Statistical significance was determined by a t -test and is indicated by * P < 0.05, ** P < 0.01, and *** P < 0.001. Data are expressed as means ± SD. ( F ) HEK293FT cells were transfected with control plasmid (−) or plasmid encoding Myc-tagged CerS4 (+) and were infected with influenza B/Lee/40 virus (IBV) or A/Hong Kong/8/68 <t>(H3N2)</t> at an MOI of 1 at 18 hours post-transfection. At 24 hpi, cells were harvested, and Western blotting was performed to detect the levels of IBV NP, IAV NS1, Myc-tagged CerS4, and GAPDH. The data are representative of three independent experiments.
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    Transient overexpression of CerS4 impairs productive infection of influenza viruses. ( A ) HEK293FT cells were transfected with control plasmid (−) or plasmid encoding Myc-tagged CerS4 (+). The cells were mock-infected or infected with IAV (pH1N1) at an MOI of 1 at 18 hours post-transfection. At 24 hpi, cells were harvested, and Western blotting was performed to detect the levels of IAV-HA, PB1, NS1, M1, Myc-tagged CerS4, and GAPDH. Densitometric values are shown, with relative expression of viral protein/GAPDH in vector control and infected samples set to 1.0. The effect of CerS4 overexpression in infected cells was compared. ( B ) A549 cells were transfected with vector control or CerS4-encoding plasmid. At 18 hours after transfections, cells were mock-infected or infected with pH1N1 at an MOI of 3 for 8 hours. Expression levels of viral proteins were assessed as shown in panel ( A ). ( C–E ) A549 cells were transfected with a vector control plasmid (Control) or plasmid encoding CerS4 (CerS4) or plasmid encoding CerS1 (CerS1, only). In ( C ), at 18 hours post-transfection, the cells were infected with IAV pH1N1 at an MOI of 0.01. At 1 ( n = 7/group), 2 ( n = 3/group), or 3 ( n = 6/group) dpi, cellular supernatants were harvested. Control vs CerS1 and Control vs CerS4 titers were statistically analyzed as indicated. In ( D ), A549 cells were infected with IAV pH1N1 at an MOI of 3 for 8 hours, followed by supernatant collection for titration by plaque assay ( n = 3/group). In ( E ), A549 cells were infected with IAV WSN at an MOI of 0.001. Cell supernatants were collected at 2 and 3 dpi for assessing viral titers using plaque assay on MDCK cells ( n = 3/group). Statistical significance was determined by a t -test and is indicated by * P < 0.05, ** P < 0.01, and *** P < 0.001. Data are expressed as means ± SD. ( F ) HEK293FT cells were transfected with control plasmid (−) or plasmid encoding Myc-tagged CerS4 (+) and were infected with influenza B/Lee/40 virus (IBV) or A/Hong Kong/8/68 <t>(H3N2)</t> at an MOI of 1 at 18 hours post-transfection. At 24 hpi, cells were harvested, and Western blotting was performed to detect the levels of IBV NP, IAV NS1, Myc-tagged CerS4, and GAPDH. The data are representative of three independent experiments.
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    Transient overexpression of CerS4 impairs productive infection of influenza viruses. ( A ) HEK293FT cells were transfected with control plasmid (−) or plasmid encoding Myc-tagged CerS4 (+). The cells were mock-infected or infected with IAV (pH1N1) at an MOI of 1 at 18 hours post-transfection. At 24 hpi, cells were harvested, and Western blotting was performed to detect the levels of IAV-HA, PB1, NS1, M1, Myc-tagged CerS4, and GAPDH. Densitometric values are shown, with relative expression of viral protein/GAPDH in vector control and infected samples set to 1.0. The effect of CerS4 overexpression in infected cells was compared. ( B ) A549 cells were transfected with vector control or CerS4-encoding plasmid. At 18 hours after transfections, cells were mock-infected or infected with pH1N1 at an MOI of 3 for 8 hours. Expression levels of viral proteins were assessed as shown in panel ( A ). ( C–E ) A549 cells were transfected with a vector control plasmid (Control) or plasmid encoding CerS4 (CerS4) or plasmid encoding CerS1 (CerS1, only). In ( C ), at 18 hours post-transfection, the cells were infected with IAV pH1N1 at an MOI of 0.01. At 1 ( n = 7/group), 2 ( n = 3/group), or 3 ( n = 6/group) dpi, cellular supernatants were harvested. Control vs CerS1 and Control vs CerS4 titers were statistically analyzed as indicated. In ( D ), A549 cells were infected with IAV pH1N1 at an MOI of 3 for 8 hours, followed by supernatant collection for titration by plaque assay ( n = 3/group). In ( E ), A549 cells were infected with IAV WSN at an MOI of 0.001. Cell supernatants were collected at 2 and 3 dpi for assessing viral titers using plaque assay on MDCK cells ( n = 3/group). Statistical significance was determined by a t -test and is indicated by * P < 0.05, ** P < 0.01, and *** P < 0.001. Data are expressed as means ± SD. ( F ) HEK293FT cells were transfected with control plasmid (−) or plasmid encoding Myc-tagged CerS4 (+) and were infected with influenza B/Lee/40 virus (IBV) or A/Hong Kong/8/68 <t>(H3N2)</t> at an MOI of 1 at 18 hours post-transfection. At 24 hpi, cells were harvested, and Western blotting was performed to detect the levels of IBV NP, IAV NS1, Myc-tagged CerS4, and GAPDH. The data are representative of three independent experiments.
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    Transient overexpression of CerS4 impairs productive infection of influenza viruses. ( A ) HEK293FT cells were transfected with control plasmid (−) or plasmid encoding Myc-tagged CerS4 (+). The cells were mock-infected or infected with IAV (pH1N1) at an MOI of 1 at 18 hours post-transfection. At 24 hpi, cells were harvested, and Western blotting was performed to detect the levels of IAV-HA, PB1, NS1, M1, Myc-tagged CerS4, and GAPDH. Densitometric values are shown, with relative expression of viral protein/GAPDH in vector control and infected samples set to 1.0. The effect of CerS4 overexpression in infected cells was compared. ( B ) A549 cells were transfected with vector control or CerS4-encoding plasmid. At 18 hours after transfections, cells were mock-infected or infected with pH1N1 at an MOI of 3 for 8 hours. Expression levels of viral proteins were assessed as shown in panel ( A ). ( C–E ) A549 cells were transfected with a vector control plasmid (Control) or plasmid encoding CerS4 (CerS4) or plasmid encoding CerS1 (CerS1, only). In ( C ), at 18 hours post-transfection, the cells were infected with IAV pH1N1 at an MOI of 0.01. At 1 ( n = 7/group), 2 ( n = 3/group), or 3 ( n = 6/group) dpi, cellular supernatants were harvested. Control vs CerS1 and Control vs CerS4 titers were statistically analyzed as indicated. In ( D ), A549 cells were infected with IAV pH1N1 at an MOI of 3 for 8 hours, followed by supernatant collection for titration by plaque assay ( n = 3/group). In ( E ), A549 cells were infected with IAV WSN at an MOI of 0.001. Cell supernatants were collected at 2 and 3 dpi for assessing viral titers using plaque assay on MDCK cells ( n = 3/group). Statistical significance was determined by a t -test and is indicated by * P < 0.05, ** P < 0.01, and *** P < 0.001. Data are expressed as means ± SD. ( F ) HEK293FT cells were transfected with control plasmid (−) or plasmid encoding Myc-tagged CerS4 (+) and were infected with influenza B/Lee/40 virus (IBV) or A/Hong Kong/8/68 <t>(H3N2)</t> at an MOI of 1 at 18 hours post-transfection. At 24 hpi, cells were harvested, and Western blotting was performed to detect the levels of IBV NP, IAV NS1, Myc-tagged CerS4, and GAPDH. The data are representative of three independent experiments.
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    Transient overexpression of CerS4 impairs productive infection of influenza viruses. ( A ) HEK293FT cells were transfected with control plasmid (−) or plasmid encoding Myc-tagged CerS4 (+). The cells were mock-infected or infected with IAV (pH1N1) at an MOI of 1 at 18 hours post-transfection. At 24 hpi, cells were harvested, and Western blotting was performed to detect the levels of IAV-HA, PB1, NS1, M1, Myc-tagged CerS4, and GAPDH. Densitometric values are shown, with relative expression of viral protein/GAPDH in vector control and infected samples set to 1.0. The effect of CerS4 overexpression in infected cells was compared. ( B ) A549 cells were transfected with vector control or CerS4-encoding plasmid. At 18 hours after transfections, cells were mock-infected or infected with pH1N1 at an MOI of 3 for 8 hours. Expression levels of viral proteins were assessed as shown in panel ( A ). ( C–E ) A549 cells were transfected with a vector control plasmid (Control) or plasmid encoding CerS4 (CerS4) or plasmid encoding CerS1 (CerS1, only). In ( C ), at 18 hours post-transfection, the cells were infected with IAV pH1N1 at an MOI of 0.01. At 1 ( n = 7/group), 2 ( n = 3/group), or 3 ( n = 6/group) dpi, cellular supernatants were harvested. Control vs CerS1 and Control vs CerS4 titers were statistically analyzed as indicated. In ( D ), A549 cells were infected with IAV pH1N1 at an MOI of 3 for 8 hours, followed by supernatant collection for titration by plaque assay ( n = 3/group). In ( E ), A549 cells were infected with IAV WSN at an MOI of 0.001. Cell supernatants were collected at 2 and 3 dpi for assessing viral titers using plaque assay on MDCK cells ( n = 3/group). Statistical significance was determined by a t -test and is indicated by * P < 0.05, ** P < 0.01, and *** P < 0.001. Data are expressed as means ± SD. ( F ) HEK293FT cells were transfected with control plasmid (−) or plasmid encoding Myc-tagged CerS4 (+) and were infected with influenza B/Lee/40 virus (IBV) or A/Hong Kong/8/68 <t>(H3N2)</t> at an MOI of 1 at 18 hours post-transfection. At 24 hpi, cells were harvested, and Western blotting was performed to detect the levels of IBV NP, IAV NS1, Myc-tagged CerS4, and GAPDH. The data are representative of three independent experiments.
    Tc Adapted Atcc Vr, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Transient overexpression of CerS4 impairs productive infection of influenza viruses. ( A ) HEK293FT cells were transfected with control plasmid (−) or plasmid encoding Myc-tagged CerS4 (+). The cells were mock-infected or infected with IAV (pH1N1) at an MOI of 1 at 18 hours post-transfection. At 24 hpi, cells were harvested, and Western blotting was performed to detect the levels of IAV-HA, PB1, NS1, M1, Myc-tagged CerS4, and GAPDH. Densitometric values are shown, with relative expression of viral protein/GAPDH in vector control and infected samples set to 1.0. The effect of CerS4 overexpression in infected cells was compared. ( B ) A549 cells were transfected with vector control or CerS4-encoding plasmid. At 18 hours after transfections, cells were mock-infected or infected with pH1N1 at an MOI of 3 for 8 hours. Expression levels of viral proteins were assessed as shown in panel ( A ). ( C–E ) A549 cells were transfected with a vector control plasmid (Control) or plasmid encoding CerS4 (CerS4) or plasmid encoding CerS1 (CerS1, only). In ( C ), at 18 hours post-transfection, the cells were infected with IAV pH1N1 at an MOI of 0.01. At 1 ( n = 7/group), 2 ( n = 3/group), or 3 ( n = 6/group) dpi, cellular supernatants were harvested. Control vs CerS1 and Control vs CerS4 titers were statistically analyzed as indicated. In ( D ), A549 cells were infected with IAV pH1N1 at an MOI of 3 for 8 hours, followed by supernatant collection for titration by plaque assay ( n = 3/group). In ( E ), A549 cells were infected with IAV WSN at an MOI of 0.001. Cell supernatants were collected at 2 and 3 dpi for assessing viral titers using plaque assay on MDCK cells ( n = 3/group). Statistical significance was determined by a t -test and is indicated by * P < 0.05, ** P < 0.01, and *** P < 0.001. Data are expressed as means ± SD. ( F ) HEK293FT cells were transfected with control plasmid (−) or plasmid encoding Myc-tagged CerS4 (+) and were infected with influenza B/Lee/40 virus (IBV) or A/Hong Kong/8/68 (H3N2) at an MOI of 1 at 18 hours post-transfection. At 24 hpi, cells were harvested, and Western blotting was performed to detect the levels of IBV NP, IAV NS1, Myc-tagged CerS4, and GAPDH. The data are representative of three independent experiments.

    Journal: Journal of Virology

    Article Title: Ceramide synthase 4 interferes with replication of influenza virus but is downregulated by infection

    doi: 10.1128/jvi.01563-25

    Figure Lengend Snippet: Transient overexpression of CerS4 impairs productive infection of influenza viruses. ( A ) HEK293FT cells were transfected with control plasmid (−) or plasmid encoding Myc-tagged CerS4 (+). The cells were mock-infected or infected with IAV (pH1N1) at an MOI of 1 at 18 hours post-transfection. At 24 hpi, cells were harvested, and Western blotting was performed to detect the levels of IAV-HA, PB1, NS1, M1, Myc-tagged CerS4, and GAPDH. Densitometric values are shown, with relative expression of viral protein/GAPDH in vector control and infected samples set to 1.0. The effect of CerS4 overexpression in infected cells was compared. ( B ) A549 cells were transfected with vector control or CerS4-encoding plasmid. At 18 hours after transfections, cells were mock-infected or infected with pH1N1 at an MOI of 3 for 8 hours. Expression levels of viral proteins were assessed as shown in panel ( A ). ( C–E ) A549 cells were transfected with a vector control plasmid (Control) or plasmid encoding CerS4 (CerS4) or plasmid encoding CerS1 (CerS1, only). In ( C ), at 18 hours post-transfection, the cells were infected with IAV pH1N1 at an MOI of 0.01. At 1 ( n = 7/group), 2 ( n = 3/group), or 3 ( n = 6/group) dpi, cellular supernatants were harvested. Control vs CerS1 and Control vs CerS4 titers were statistically analyzed as indicated. In ( D ), A549 cells were infected with IAV pH1N1 at an MOI of 3 for 8 hours, followed by supernatant collection for titration by plaque assay ( n = 3/group). In ( E ), A549 cells were infected with IAV WSN at an MOI of 0.001. Cell supernatants were collected at 2 and 3 dpi for assessing viral titers using plaque assay on MDCK cells ( n = 3/group). Statistical significance was determined by a t -test and is indicated by * P < 0.05, ** P < 0.01, and *** P < 0.001. Data are expressed as means ± SD. ( F ) HEK293FT cells were transfected with control plasmid (−) or plasmid encoding Myc-tagged CerS4 (+) and were infected with influenza B/Lee/40 virus (IBV) or A/Hong Kong/8/68 (H3N2) at an MOI of 1 at 18 hours post-transfection. At 24 hpi, cells were harvested, and Western blotting was performed to detect the levels of IBV NP, IAV NS1, Myc-tagged CerS4, and GAPDH. The data are representative of three independent experiments.

    Article Snippet: Both the A/Hong Kong/8/68 (H3N2) virus (ATCC VR-1679) and the B/Lee/40 (IBV) virus (ATCC VR-1535) were purchased through ATCC.

    Techniques: Over Expression, Infection, Transfection, Control, Plasmid Preparation, Western Blot, Expressing, Titration, Plaque Assay, Virus